Articles
This article gives a most useful overview of the analysis and sequencing of nucleic acids by matrix-assisted laser desorption/ionisation mass spectrometry, which should interest all readers and may well serve as a useful tutorial article.
After an introduction to MALDI, DESI and SIMS, the authors describe their main applications in forensics, as well as the advantages provided in terms of sample preparation over approaches routinely used in toxicological laboratories.
This article shows that MALDI high resolution mass spectrometry demonstrated directly that Hev b 6 and Hev b 1, and also truncated forms thereof, are present on the inner surface of medical NRL gloves. The speed and high accuracy of the applied method and instrumentation makes the detection of surface associated proteins feasible without any prior protein extraction procedure (in situ localisation).
In 2007 we introduced our method in Spectroscopy Europe describing the identification of furs by hair digest based matrix-assisted laser desorption/ionisation time of flight (MALDI-ToF) mass spectrometry (MS). This so-called SIAM (Species Identification of Animals) method can also be used for the species determination of ancient furs.
Maria C. Prieto Conaway,a Shousong Cao,b Farukh Durrani,b Youcef Rustum,b Ping Wang,c Khin Marlarc and Latif Kazimc
aThermo Fisher Scientific, San Jose, CA, USA
bRoswell Park Cancer Institute, Department of Cancer Biology, Buffalo, NY, USA
cRoswell Park Cancer Institute, Department of Cell Stress Biology, Buffalo, NY, USA
The authors show how the use of SIAM method (Species Identification of Animals MALDI-TOF mass spectrometry) is a fast and reliable tool for recognising the origin of mammalian species.
