Water is the Earth’s most abundant natural resource, but it’s also something of a mystery due to its unique solvation characteristics. How water relates to and interacts with biological systems, like DNA, is of critical importance, and the group of Poul Petersen, assistant professor of chemistry and chemical biology at Cornell University, has used a relatively new form of spectroscopy to observe a previously unknown characteristic of water.
This work has been published in ACS Central Science, and reports the first observation of a chiral water superstructure surrounding a biomolecule. In this case, the water structure follows the iconic helical structure of DNA, which itself is chiral, meaning it is not superimposable on its mirror image. Chirality is a key factor in biology, because most biomolecules and pharmaceuticals are chiral.
“If you want to understand reactivity and biology, then it’s not just water on its own”, Petersen said. “You want to understand water around stuff, and how it interacts with the stuff. And particularly with biology, you want to understand how it behaves around biological material—like protein and DNA.”
Water plays a major role in DNA’s structure and function, and its hydration shell has been the subject of much study. Molecular dynamics simulations have shown a broad range of behaviours of the water structure in DNA’s minor groove, the area where the backbones of the helical strand are close together.
The group’s work employed chiral sum frequency generation (SFG) spectroscopy, a technique Petersen detailed in 2015 in the Journal of Physical Chemistry. SFG is a non-linear optical method in which two photon beams, one infrared and one visible, interact with the sample, producing an SFG beam containing the sum of the two beams’ frequencies. In this case, the sample was a strand of DNA linked to a silicon-coated prism. More manipulation of the beams and calculation proved the existence of a chiral water superstructure surrounding DNA.
In addition to the novelty of observing a chiral water structure template by a biomolecule, chiral SFG provides a direct way to examine water in biology.
“The techniques we have developed provide a new avenue to study DNA hydration, as well as other supramolecular chiral structures”, Petersen said.
The group admits that their finding’s biological relevance is unclear, but Petersen thinks the ability to directly examine water and its behaviour within biological systems is important.
“Certainly, chemical engineers who are designing biomimetic systems and looking at biology and trying to find applications such as water filtration would care about this,” he said.
Another application, Petersen said, could be in creating better anti-biofouling materials, which are resistant to the accumulation of microorganisms, algae and the like on wetted surfaces.
