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Imaging

Raman spectroscopy in the characterisation of carious dental tissues

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Photo of caries in human teeth.

In this work we demonstrate the suitability of confocal Raman microscopy for the characterisation of carious dental tissues. Samples of enamel and dentine, presenting carious lesions in different stages of progression, were evaluated by comparing the depolarisation ratio of the PO43– symmetric stretching band at 959 cm–1 in the different tissues. Both line and area scans were performed to gauge these variations. Moreover, the obtained results were compared with the tissues’ behaviour when interacting with ultraviolet radiation, namely the induced fluorescence in some tissues. The depolarisation ratio has proven to be a valuable tool in recognition of demineralisation of both enamel and dentine due to caries. The analyses of the collagen bands in the dentine sample turned out to be more difficult to evaluate due to high fluorescence in the carious region.

TrueSurface

Next generation of WITec’s optical profilometer which has improvements in ease of use, accelerated workflow and methodological advantages. TrueSurface ensures that Raman spectra are acquired precisely along a surface or at a set, user-defined, distance from a surface.

Suite Five

Software and hardware improvements for Raman scanning-probe microscopes. A new software wizard guides the user through the complete investigation, from initial settings and acquisition through data and image post-processing. Presets and highlighted analytical paths speed up the generation of images. TrueComponent Analysis is a post-processing function for confocal Raman imaging measurements that automatically establishes the number of components in a sample, locates them in the image and differentiates their individual spectra.

EasyNav Software

Contains three separate applications for navigating and acquiring Raman images. NavMap is a video feature that shows the whole sample and the zoomed sample’s image in the region of interest simultaneously. NavSharp enables sharp and rapid navigation on a sample image with any topography, keeping the field of view constantly focussed on the sample surface. View Sharp constructs an image in which all surfaces are in focus simultaneously, and creates a 3D topographical image.

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