Informing Spectroscopists for Over 40 Years

Articles

Intrinsic fluorescence of proteins as a medical diagnostic tool

Dmitry Gakamsky and Anna Gakamsky describe how fluorescence may be used to diagnose cataracts in human eye lenses. Further, it may also be able to grade cataracts and monitor the disease’s progress, which may help discover metabolic and ambient factors that influence the progress of the disease.

The CAL(AI)2DOSCOPE: a microspectrophotometer for accurate recording of correlated absorbance and fluorescence emission spectra

The CAL(AI)2DOSCOPE (Cryogenic Absorption/Luminescence Alignment Independent Alternative Intermittent Detection Optical µSCOPE) is a microspectrometer that was constructed with the aim to facilitate the correlated investigation of absorption and fluorescence emission properties of nanovolumic protein samples under modulatable actinic illumination.

Glowing colours of foods: application of fluorescence and chemometrics in food studies

Whilst the major components of food are usually non-fluorescent, many minor food components that affect its nutritive, compositional and technological quality are fluorescent. Given its sensitivity, ease of use and non-destructive nature, this makes it useful in many applications around monitoring food processing and in fundamental food research.

Application of Raman and photoluminescence spectroscopy for identification of uranium minerals in the environment

The “Application of Raman and photoluminescence spectroscopy for identification of uranium minerals in the environment” is described by Eric Faulques, Florian Massuyeau, Nataliya Kalashnyk and Dale Perry. Uranium forms a large number of compounds and complexes, and these are most helpful in the study of uranium, its chemistry and transport in the environment. Raman and photoluminescence spectroscopy provide complementary information and are powerful tools for direct speciation of uranium and identification of natural uranyl minerals relevant to the environment.

Optical spectroscopy in therapy response monitoring: an awakening giant

“Optical spectroscopy in therapy response monitoring: an awakening giant” by Arja Kullaa, Surya Singh, Jopi Mikkonen and Arto Koistinen looks at the important advances made by optical spectroscopy techniques, such as diffuse optical spectroscopic imaging (DOSI), Raman, diffuse reflectance and fluorescence spectroscopy, in changing how cancer is managed in patients. The ability to repeatedly monitor tumour dynamics to see how effective a particular treatment has been has enormous potential for us all.

Orthogonal spectroscopic techniques for the early developability assessment of therapeutic protein candidates

Orthogonal spectroscopic techniques for the early developability assessment of therapeutic protein candidates” are described by Patrick Garidel, Anne Karow and Michaela Blech. Due to its cost and time implications, in the early development phase of drug discovery the use of  othogonal techniques, based on different physical observables, is important for correct decision-making.

On-chip chemical fingerprinting of an analyte using both Raman spectroscopy and fluorescence

Praveen Ashok and Kishan Dholakia of St Andrews University, UK, describe the scope of optofluidic devices that can be implemented using the waveguide confined Raman spectroscopy (WCRS) technique they have developed. I am particularly impressed by the sample size of whisky shown in Figure 2—true Scottish style!

Right angle light scattering protein thermostability screening: from research to development

In the pharmaceutical industry, biologicals are of increasing interest due to their high therapeutic benefits. Amongst many other questions, the stability of new canditates is of great importance. Patrick Garidel describes this in “Right angle light scattering protein thermostability screening: from research to development”. The ability of this and other fluorescence-based techniques to detect very small quantities is of great benefit.

Complementary spectroscopic analyses of varnishes of historical musical instruments

Jean-Philippe Echarda and Loïc Bertrandb

aLaboratoire de recherche et de restauration, Musée de la musique, Cité de la musique, 221 avenue Jean Jaurès, 75019 Paris, France. E-mail: jpechard@cite-musique.fr
bIPANEMA, synchrotron SOLEIL, Saint-Aubin, 91192 Gif-sur-Yvette cedex, France

Steady-state intrinsic tryptophan protein fluorescence spectroscopy in pharmaceutical biotechnology

Over the last two decades therapeutic antibodies have become the fastest growing area in pharmaceutical biotechnology. The medical significance of these therapeutic entities is highlighted by the commercial availability of about 20 products on the market with more than 160 candidates evaluated in different clinical trials. One reason for the success of antibodies as therapeutic agents is related to the large advancement in their biotechnological production via fermentation. Nowadays titers of about 4 g L–1 in 11-day fed-batch mode using the CHO BI HEX process are achievable using CHO-cells (CHO: chinese hamster ovary).

Understanding and measuring photooxidation in dairy products by fluorescence spectroscopy

Jens Petter Wold

MATFORSK, Norwegian Food Research Institute, Osloveien 1, 1430 Ås, Norway

Trilinear fluorescence spectroscopy

Mikael Kubista, Jahan Ghasemi, Björn Sjögreen and Amin Forootan

MultiD Analyses AB, Göteborg, Sweden. E-mail: info@multid.se